Evaluation of Acute 13-Week Subchronic Toxicity and Genotoxicity of the Powdered Root of Tongkat Ali (Eurycoma longifolia Jack)

Table 4

Hematology analysis of subchronic toxicity after 13-week E. longifolia powdered root treatment.

Item (abbreviation)	Unit	Treatment (g/kg b.w.)
		Male				Female
		0	0.6	1.2	2	0	0.6	1.2	2
Red blood cell count (RBC)	10^6/μL	8.20±0.38	8.26±0.41	8.44±0.38	8.41±0.30	7.98±0.17	7.91±0.42	7.77±0.37	8.37±0.35
Hematocrit (HCT)	%	43.88±1.27	43.78±1.88	43.88±2.23	43.01±1.34	44.63±1.42	43.94±2.54	43.37±3.06	44.78±2.08
Hemoglobin (Hb)	g/dL	14.29±0.46	14.33±0.64	14.63±0.79	14.52±0.39	14.49±0.47	14.40±0.86	14.40±0.85	15.02±0.67
Mean corpuscular hemoglobin (MCH)	pg	17.43±0.53	17.37±0.41	17.33±0.43	17.29±0.55	18.16±0.59	18.19±0.54	18.52±0.51	17.94±0.32
MCH concentration (MCHC)	g/dL	32.57±0.50	32.74±0.65	33.36±0.61^∗	33.76±0.39^∗	32.47±0.68	32.78±0.77	33.24±1.08	33.56±0.74
Mean corpuscular volume (MCV)	fL	53.56±1.62	53.06±1.64	52.02±1.26	51.20±2.04^∗	55.95±2.15	55.55±1.62	55.79±2.88	53.50±1.21
Red cell distribution width (RDW)	fL	25.81±2.05	25.01±1.47	24.37±1.32	24.26±1.09	26.48±1.83	25.89±1.77	26.23±1.92	24.52±1.66
Mean platelet volume (MPV)	fL	7.70±0.21	7.76±0.23	7.48±0.21	7.49±0.24	8.37±0.45	8.16±0.51	8.23±0.43	8.01±0.46
Platelet distribution width (PDW)	fL	8.26±0.36	8.32±0.34	7.87±0.47	7.84±0.37	9.50±0.73	9.15±0.95	9.29±0.66	9.06±0.87
White blood cell count (WBC)	10^3/μL	2.62±0.94	3.46±1.18	4.44±3.27	3.09±1.59	2.03±0.95	1.72±1.20	1.61±0.99	3.37±1.40
Lymphocytes	10^3/mm^3	67.41±10.10	67.71±6.04	70.17±15.34	69.97±7.34	70.12±12.07	66.23±12.91	73.24±5.40	76.94±8.13
Neutrophils	10^3/mm^3	26.32±9.82	8.32±0.34	7.87±0.47	7.84±0.37	22.07±7.87	27.98±11.38	21.31±5.20	17.83±6.74
Monocytes	10^3/mm^3	3.24±2.69	4.31±2.40	3.03±2.45	2.86±2.05	4.76±6.63	2.18±1.96	3.25±2.43	2.95±1.35
Eosinophil	10^3/mm^3	3.03±1.21	2.50±0.46	2.42±1.29	2.29±0.76	3.05±1.14	3.61±2.02	2.20±1.84	2.28±1.11
Basophils	10^3/mm^3	0.00±0.00	0.00±0.00	0.02±0.06	0.00±0.00	0.00±0.00	0.00±0.00	0.00±0.00	0.00±0.00
Platelets count (PLT)	10^3/μL	635.9±67.6	678.2±66.3	732.6±97.9	729.0±89.1	752.3±65.5	684.4±115.9	845.7±110.3	773.4±108.6
Prothrombin time (PT)	Sec	12.33±1.23	12.51±1.05	11.11±0.84^∗	10.59±0.19^∗	10.34±0.72	10.36±0.42	10.87±0.99	10.68±0.47
Partial thromboplastin time (APTT)	Sec	32.24±5.00	32.45±6.62	27.94±3.07^∗	23.43±2.32^∗	29.51±4.84	30.39±5.12	31.08±3.65	31.16±3.69
Fibrinogen (Fbg)	mg/dL	183.4±27.6	185.0±36.4	176.7±35.5	168.6±36.9	126.3±13.6	125.1±18.6	125.6±10.6	128.3±16.4

Hematology profiles were within normal range (Charles River, 2008) and unaffected by the E. longifolia powdered root ingestions. Data were expressed as Mean ± S.D. (𝑁=8--10). Statistical analysis: ^∗𝑃<0.05 indicates a statistical difference with the control group by one-way ANOVA.