Cornel Iridoid Glycoside Attenuates Tau Hyperphosphorylation by Inhibition of PP2A Demethylation

Figure 2

CIG protects microtubular cytoskeleton against injury induced by WT/GFX in SK-N-SH cells. Antibodies to 𝛽-tubulin and confocal fluorescence microscopy were used to visualize microtubular cytoskeleton. Control: normal human neuroblastoma SK-N-SH cells; Con + CIG: 100 μg/mL CIG was incubated with normal SK-N-SH cells for 24 h; WT/GFX: SK-N-SH cells were exposed to 10 μM WT/GFX for 3 h (the arrows point to the damaged microtubule cytoskeleton); WT/GFX + CIG (L, M, and H): SK-N-SH cells were preincubated with CIG (50, 100, and 200 μg/mL) for 24 h and then exposed to 10 μM WT/GFX for 3 h after washing out CIG. Bar = 50 μm.