Baicalin’s Therapeutic Time Window of Neuroprotection during Transient Focal Cerebral Ischemia and Its Antioxidative Effects In Vitro and In Vivo
Figure 4
Effects of baicalin on the oxidative stress in mice undergoing a middle cerebral artery occlusion. Twenty-four hours after the onset of MCAO, the ROS were detected in regions of the prefrontal cortex using DHE staining. The relative intensity of the red fluorescence represents the ROS content in the prefrontal cortex region of the injured hemisphere in the prefrontal cortex, (A) sham group, (B) ischemia group, and (C) baicalin treatment group ((a), DHE staining ×200). The relative fluorescence intensity in 5 sites obtained from one section was determined by fluorescence microscopy. The mean value of the ROS content was calculated and expressed as the mean ± SEM, (b). The MDA content, NADPH oxidase activity, and SOD activity are shown in (c), (d), and (e), respectively (). ΔΔ, ischemia group versus sham; **, baicalin-treated group versus ischemia group.
