Fermented Wheat Germ Extract Induced Cell Death and Enhanced Cytotoxicity of Cisplatin and 5-Fluorouracil on Human Hepatocellular Carcinoma Cells
Figure 2
FWGE-induced cell death in HCC cells. (a) HepG2, Hep3B, and HepJ5 cells were treated by FWGE for 72 hr. Survival cells were determined by size measurements for the cells sized between 10 to 22 m. (a) Survival cell number of FWGE treated HepG2, Hep3B, and HepJ5 cells after 72 hr. All cell lines showed dose-dependent effects following FWGE treatment (one-way ANOVA, on HepG2 and Hep3B cells and on HepJ5 cells). Experiments were performed in triplicate at least and data were shown as mean ± SD. (b) Cell size distribution of cells in control medium or (c) FWGE (0.5, 0.4 and 1.0 mg/mL in HepG2, Hep3B, and HepJ5 cells for 72 hr) treated cells.
