Flavonoids Induce the Synthesis and Secretion of Neurotrophic Factors in Cultured Rat Astrocytes: A Signaling Response Mediated by Estrogen Receptor
Figure 3
Flavonoids increase the mRNA levels of NGF, GDNF, and BDNF in cultured astrocytes in dose-dependent manner. The flavonoids calycosin, isorhamnetin, luteolin and genistein (1, 3, 10 M) were applied to cultured astrocytes for 48 hours. 17-estradiol at 100 nM served as the positive control. The mRNA levels of NGF, GDNF, and BDNF were measured by real-time quantitative PCR. Values are expressed as the fold of change ( Basal) against the control (no treatment; set as 1), and in Mean SEM, , each with triplicate samples. where compared to the control.
