Honokiol Eliminates Human Oral Cancer Stem-Like Cells Accompanied with Suppression of Wnt/β-Catenin Signaling and Apoptosis Induction
Figure 4
Honokiol-induced apoptosis of SAS SP cells. (a) Annexin V-PI staining of SP cells after treatment with honokiol for 48 h. Q1: quadrant (annexin V−, PI+) represents dead cells; Q2: quadrant (annexin V+, PI+) represents late apoptotic cells; Q3: quadrant (annexin V+, PI–) represents early apoptotic cells; Q4: quadrant (annexin V−, PI−) represents live cells. The percentage of cell population in each quadrant is calculated and shown in histograms and a dose-dependent increase of honokiol-induced apoptosis was displayed. (b) Decrease of Survivin and Bcl-2 protein levels and increase of caspase-3 cleavage in honokiol-treated SP cells. Cells were treated with honokiol for 48 h and the lysates were analyzed by Western blot using β-actin as loading control.
