Attenuation of Proinflammatory Responses by S-[6]-Gingerol via Inhibition of ROS/NF-Kappa B/COX2 Activation in HuH7 Cells
Figure 3
S-[6]-Gingerol inhibits IL1β-induced oxidative stress in HuH7 cells. HuH7 cells were pretreated with 100 μM S-[6]-gingerol (G) or DMSO (vehicle control) for 6 h then stimulated with IL1β (8 ng/mL) for 3 h. (a) The levels of ROS generation were measured using the NBT reduction assay. The relative concentration of ROS was calculated based on the generated amount of formazan. (b) The mRNA levels of DHCR24 were measured using RT-qPCR, normalised to B2M. Results are expressed as mean ± SEM of 3 independent experiments, relative to DMSO controls. versus DMSO vehicle control; versus IL1β treatment.
