Attenuation of Proinflammatory Responses by S-[6]-Gingerol via Inhibition of ROS/NF-Kappa B/COX2 Activation in HuH7 Cells
Figure 4
S-[6]-Gingerol suppresses IL1β-induced COX2 expression and NFκB activity in IL1β-stimulated HuH7 cells. (a) HuH7 cells were pretreated with 100 μM S-[6]-gingerol (G) or DMSO (vehicle control) for 6 h then stimulated with IL1β (8 ng/mL) for 3 h. The mRNA levels of COX2 were measured using RT-qPCR, normalised to B2M. (b) HuH7 cells were transfected with the NFκB-luciferase reporter vector. Transfectants were preincubated for 6 h with 100 μM S-[6]-gingerol (G) or DMSO (vehicle control) and then stimulated with IL1β for 3 h. Cells were then harvested and cell lysates assayed for luciferase activity. Results are expressed as mean ± SEM of 3 independent experiments, relative to the DMSO vehicle controls. versus DMSO vehicle control; versus IL1β treatment.
