Attenuation of Proinflammatory Responses by S-[6]-Gingerol via Inhibition of ROS/NF-Kappa B/COX2 Activation in HuH7 Cells
Figure 5
Comparison of the effects of S-[6]-gingerol with those of selective inhibitors on increased IL6, IL8, and SAA1 expression induced by IL1β in HuH7 cells. Before treatment with IL1β (8 ng/mL) for 3 h, HuH7 cells were pretreated with 100 μM S-[6]-gingerol (G) for 6 h, or with 100 μM NS398, 50 μM PDTC, or 50 μM BHT for 30 min. The mRNA levels of IL6 (a), IL8 (b), and SAA1 (c) were detected using RT-qPCR, normalized to B2M. Results are expressed as mean ± SEM of 3 independent experiments, relative to DMSO controls. *P < 0.05 versus DMSO vehicle control; versus IL1β treatment.
