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Evidence-Based Complementary and Alternative Medicine
Volume 2013 (2013), Article ID 154606, 9 pages
Research Article

Phytochemicals and Antioxidant Capacity from Nypa fruticans Wurmb. Fruit

1Chemical Engineering Discipline, School of Engineering, Monash University, Jalan Lagoon Selatan, 46150 Bandar Sunway, Selangor, Malaysia
2Department of Nutrition and Dietetics, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia
3Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China
4Department of Molecular Medicine, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia
5Institute of Agro-food Science and Technology, Guangxi Academy of Agricultural Sciences, Nanning 530007, China
6Laboratory of Halal Science Research, Halal Products Research Institute, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia
7MUDA Agricultural Development Authority (MADA), Alor Setar 05990, Kedah, Malaysia

Received 9 October 2012; Revised 20 February 2013; Accepted 18 March 2013

Academic Editor: Weena Jiratchariyakul

Copyright © 2013 Nagendra Prasad et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Nypa fruticans Wurmb. is one of the important underutilized fruit of Malaysia, which lacks scientific attention. Total phenolics, flavonoid content, and antioxidant capacities from endosperm extracts of Nypa fruticans (unripe and ripe fruits) were evaluated. Endosperm extract of unripe fruits (EEU) exhibited the highest phenolics ( mg GAE/g), flavonoid content ( RE/g), and antioxidant capacity. Free radical scavenging capacity of EEU as assessed by 2--azino-bis (3-ethylbenz-thiazoline-6-sulfonic acid (ABTS) and 1,1-diphenyl-2-picryl hydrazyl (DPPH) radicals showed inhibitory activity of % and %, respectively. Beta carotene bleaching coefficient of EEU was higher (), when compared to endosperm extract of ripe fruits (). Additionally, EEU exhibited high antioxidant capacity by phosphomolybdenum method and ferric reducing antioxidant power values. Eight phenolic compounds from Nypa fruticans endosperm extracts were identified and quantified by ultra-high-performance liquid chromatography. Chlorogenic acid, protocatechuic acid, and kaempferol were the major phenolic compounds. Thus this fruit could be used as a potential source of natural antioxidant.