Intestinal Protective Effects of Herbal-Based Formulations in Rats against Neomycin Insult

Table 3

Primer sequences used for the detection of colonic expression of key inflammatory mediators and cytokines as well as tight junction proteins and MUC-2 in rats using qRT-PCR.

Target gene	PS	Sequence (5′–3′)	OAT	References
Claudin-1	F	TGTAATTTCAGGTCTGGCGACA	53°C	[19]
	R	GGATAAGGCCGTGGTGTTGG
COX-2	F	CTCTGCGATGCTCTTCCGAG	48°C	[20]
	R	AAGGATTTGCTGCATGGCTG
GAPDH	F	ATGGCACAGTCAAGGCTGAGA	53°C	[21]
	R	CGCTCCTGGAAGATGGTGAT
ICAM-1	F	CGTGGCGTCCATTTACACCT	58°C	[21]
	R	TTAGGGCCTCCTCCTGAGC
IL-1β	F	CACCTCTCAAGCAGAGCACAG	53°C	[22]
	R	GGGTTCCATGGTGAAGTCAAC
IL-6	F	GCCCTTCAGGAACAGCTATGA	55°C	[20]
	R	TGTCAACAACATCAGTCCCAAGA
IL-10	F	TGCAACAGCTCAGCGCA	53°C	[23]
	R	GTCACAGCTTTCGAGAGACTGGAA
Occludin	F	TTACGGCTATGGAGGGTACAC	50°C	[24]
	R	TGACGCTGGTAACAAAGATCAC
MUC-2	F	GCCAGATCCCGAAACCA	50°C	[25]
	R	TATAGGAGTCTCGGCAGTCA
TNF-α	F	GGTGATCGGTCCCAACAAGGA	45°C	[26]
	R	CACGCTGGCTCAGCCACTC
ZO-1	F	TTCCGCCTCTGTCCAACTCT	53°C	[24]
	R	ATGGGGGTGGGTCTGGTTTC

PS: primer sets; F: forward; R: reverse; OAT: optimized annealing temperature.