Intestinal Protective Effects of Herbal-Based Formulations in Rats against Neomycin Insult

Table 4

Effect of oral treatment of neomycin either alone or in combination with HF-1, HF-2, and probiotic on the colonic expression of key inflammatory mediators and cytokines in rats¹.

Treatment	Level of mRNA (% of control)
	COX-2	TNF-α ^2	IL-1β ^>2	IL-6	IL-10	ICAM-1
Control	100.00 ± 15.29	100.00 ± 15.04	100.00 ± 3.91	100.00 ± 1.67	100.00 ± 3.60	100.00 ± 4.08
Neomycin	91.09 ± 7.01	86.02 ± 4.24	107.55 ± 2.29	103.86 ± 3.95	105.55 ± 5.00	97.55 ± 3.22
Neomycin + HF-1	86.99 ± 10.94	108.39 ± 1.59	109.21 ±1.35	104.37 ± 9.31	104.14 ± 3.82	94.38 ± 1.61
Neomycin + HF-2	102.55 ± 2.47	104.48 ± 1.99	105.74 ± 2.56	103.82 ± 2.53	116.05 ± 5.29	92.75 ± 2.00
Neomycin + probiotic	101.86 ± 7.48	111.43 ± 3.55	108.91 ± 7.44	103.70 ± 5.26	116.30 ± 5.47	97.97 ±3.25

1The detailed treatment regimen and experimental conditions are described in Section 2. The level of expression of genes in control group was set to 100%. Data are means ± SEM, n = 4. ²Data were log-transformed prior to analysis by ANOVA. None of the genes showed significant difference in expression between the groups.