Intestinal Protective Effects of Herbal-Based Formulations in Rats against Neomycin Insult

Table 5

Effect of oral treatment of neomycin either alone or in combination with herbal formulation 1 (HF-1), herbal formulation 2 (HF-2), and probiotic on the colonic expression of key tight junction proteins and MUC-2 in rats¹.

Treatment	Level of mRNA (% of control)
	ZO-1	Claudin-1	Occludin	MUC-2^2
Control	100.00 ± 5.70^a	100.00 ± 7.71^a,b	100.00 ± 4.08^a	100.00 ± 17.29^a
Neomycin (Neo)	65.11 ± 5.90^b	72.87 ± 3.12^c	97.11 ± 1.47^a	98.03 ± 12.16^a
Neo + HF-1	98.04 ± 8.43^a,b	80.77 ± 5.24^a,c	99.85 ± 2.60^a	213.73 ± 13.40^b
Neo + HF-2	109.38 ± 9.46^a	98.05 ± 3.68^a,d	101.35 ± 8.47^a	246.28 ± 13.41^b
Neo + probiotic	115.00 ± 5.37^a	113.98 ± 5.57^b,d	110.40 ± 5.70^a	325.29 ± 87.25^b

1The detailed treatment regimen and experimental conditions are described in Section 2. The level of expression of genes in control group was set to 100%. Data are means ± SEM, n = 4. ²Data were log-transformed prior to analysis by ANOVA. Means in a column with superscripts without a common letter differ, P < 0.05.