Evaluation of the Bronchorelaxant, Genotoxic, and Antigenotoxic Effects of Cassia alata L.

Figure 5

(a) Rat-liver comet assay: representative comet images from (A) treatment with AECal and (B) CP-treated cell (positive control). DNA was stained with propidium iodide; 400x magnification. Images were treated with the CometScore software to eliminate the background from the field of view before acquiring comet metrics. (b) Genotoxic effects of aqueous-ethanolic leaf extract of Cassia alata assessed by comet assay in rat livers cells. ^∗𝑃<0.05, ^∗∗𝑃<0.01, and ^∗∗∗𝑃<0.001 versus negative control (ANOVA followed by Tukey’s test). (c) Antigenotoxic effects of aqueous-ethanolic leaf extract of Cassia alata assessed by comet assay in rat livers cells. ^∗𝑃<0.05, ^∗∗𝑃<0.01, and ^∗∗∗𝑃<0.001 versus negative control; ^a𝑃<0.05, ^b𝑃<0.01, and ^c𝑃<0.001 versus the corresponding cyclophosphamide alone (ANOVA followed by Tukey’s test).

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