Triptolide-Mediated Apoptosis by Suppression of Focal Adhesion Kinase through Extrinsic and Intrinsic Pathways in Human Melanoma Cells

Figure 6

Effect of TPL on caspase-dependent cleavage of FAK in SK-MEL-2 cells. Cells were incubated with 100 μM of caspase inhibitors, z-IETD-FMK, z-LEHD-FMK, Ac-DMQD-FMK, or z-VAD-FMK, for 1 h and then coincubated with 50 nM of TPL for 24 h, and then cells were collected. Cell lysates were analyzed by Western blotting. Each protein was detected using caspase-3 and FAK antibodies. GAPDH was used as an internal control.