Qushi Huayu Decoction Inhibits Hepatic Lipid Accumulation by Activating AMP-Activated Protein Kinase In Vivo and In Vitro

Figure 5

Effects of QHD-containing serum on cellular steatosis in L02 cells stimulated with FFA. (A) Intracellular TG content in L02 cells incubated with different concentrations of FFA for different times. The concentrations of low, middle, and high dose of FFA were 0.25 mM oleate/0.125 mM palmitate, 0.5 mM oleate/0.25 mM palmitate, and 1 mM oleate/0.5 mM palmitate, respectively. **𝑃<0.01, versus the control group at the same time point. (B) Effects of QHD-containing serum on cell viability of L02 cells. Cell viability was determined by the alamarBlue assay. (C) Effects of different concentrations of QHD-containing serum on intracellular TG contents in L02 cells incubated with FFA. **𝑃<0.01, versus the 5% vehicle control group; ^&&𝑃<0.01, versus the 10% vehicle control group; ^##𝑃<0.01, versus the FFA + 10% vehicle control group. (D) Effect of QHD-containing serum on cellular lipid droplets in L02 cells stimulated with FFA (oil red O staining, magnification ×100). (a) Control group; (b) FFA group; (c) FFA + QHD group.