Synergistic Anticancer Effects of Vorinostat and Epigallocatechin-3-Gallate against HuCC-T1 Human Cholangiocarcinoma Cells

Figure 2

The synergistic effect of the combination of vorinostat and EGCG was assessed on apoptosis and necrosis of HuCC-T1 cholangiocarcinoma cells. (a) Flow cytometric analysis of tumor cells. FITC-Annexin V and propium iodide (PI) were used for apoptosis and necrosis analysis of tumor cells, respectively. (b) TUNEL assay. (c) Western blot assay: (A), Bax/Bcl-2 expression; (B), PARP expression; extent of caspase-3 and-7 activity. **P < 0.01.