Suppressions of Migration and Invasion by Cantharidin in TSGH-8301 Human Bladder Carcinoma Cells through the Inhibitions of Matrix Metalloproteinase-2/-9 Signaling
Figure 4
Cantharidin inhibits the migration and invasion of TSGH-8301 cells in vitro. Cells were placed in the well at the density of 5 × 104 cells/well and then were treated with 0, 1, and 2.5 μM cantharidin then that penetrated through with or without the Matrigel to the lower surface of the filter were examined. Cells were stained with crystal violet and then were examined and photographed under a light microscope at 200x (a and c). The quantification of cells from each treatment in the lower chambers was counted at 200x (b) and (d). Columns repeat the mean from three independent experiments. , significant difference between cantharidin-treated groups and the control as analyzed by Dunnett’s posttest.
