AP-1/IRF-3 Targeted Anti-Inflammatory Activity of Andrographolide Isolated from Andrographis paniculata
Figure 3
The effect of AG on mRNA expression of iNOS, COX-2, and TNF-α genes in LPS-treated RAW264.7 cells. RAW264.7 cells ( cells/mL) were incubated with AG in the absence or presence of LPS (1 μg/mL) for 6 h. mRNA levels for iNOS, COX-2, and TNF-α were determined by (a) semi-quantitative PCR and (b) by real-time PCR. GAPDH was used as an internal control. (c) The promoter activities of iNOS and COX-2 were determined by luciferase reporter gene assay in HEK293 cells as described in Section 2. Data ((b) and (c)) are presented as the mean ± SD of an experiment done with 6 biological replicates () per treatment. (a) is a representative image of three different gels with similar results. and compared to control group.
