AP-1/IRF-3 Targeted Anti-Inflammatory Activity of Andrographolide Isolated from Andrographis paniculata
Figure 7
In vivo anti-inflammatory effect of AG on LPS-induced hepatitis and HCl/EtOH-induced gastritis animal models. Mice were administered AG orally for 6 days and treated intraperitoneally with LPS or LPS/D-galactosamine to induce hepatitis. After 1 h, ((a), left panel) livers were resected and photos were taken, ((a), right panel) after which serum was prepared to measure biochemical parameters (AST and ALT). (b) The inhibitory activity of AG on the phosphorylation of transcription factor ATF2 in the livers from LPS-treated mice was examined by immunoblotting. The levels of total or phospho-forms of ATF2 were measured from whole liver lysates prepared from LPS-treated mice. (c) Mice were orally administered with AG or ranitidine for 3 days and treated orally with EtOH/HCl to induce gastritis. After 1 h, ((c), left panel) stomachs were resected and photos were taken of the gastric lesions in the stomach. The areas of gastric lesions ((c), right panel) were measured. Data ((a) right panel and (c) right panel) are presented as the mean ± SD of an experiment done with 6 mice (). compared to the control group.
