Research Article
AP-1/IRF-3 Targeted Anti-Inflammatory Activity of Andrographolide Isolated from Andrographis paniculata
Table 1
PCR primers used in this study.
| | Name | | Sequence (5′ to 3′) |
| | Real-time PCR | | | | iNOS | F | GGAGCCTTTAGACCTCAACAGA | | R | TGAACGAGGAGGGTGGTG | | IFN- | F | TCCAAGAAAGGACGAACATTCG | | R | GAGGCCATTTGGGAACTTCT | | COX-2 | F | CACTACATCCTGACCCACTT | | R | ATGCTCCTGCTTGAGTATGT | | IRF3 | F | GGCTGACTTTGGCATCTT | | R | TTCCTCTTCCAGGTTGACA | | GAPDH | F | CAATGAATACGGCTACAGCAAC | | R | AGGGAGATGCTCAGTGTTGG | | Semiquantitative PCR | | | | iNOS | F | CCCTTCCGAAGTTTCTGGCAGCAG | | R | GGCTGTCAGAGCCTCGTGGCTTTGG | | TNF- | F | TTGACCTCAGCGCTGAGTTG | | R | CCTGTAGCCCACGTCGTAGC | | IFN- | F | CAGGATGAGGACATGAGCACC | | R | CTCTGCAGACTCAAACTCCAC | | COX-2 | F | CACTACATCCTGACCCACTT | | R | ATGCTCCTGCTTGAGTATGT | | GAPDH | F | CACTCACGGCAAATTCAACGGCA | | R | GACTCCACGACATACTCAGCAC |
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