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Evidence-Based Complementary and Alternative Medicine
Volume 2013, Article ID 215391, 12 pages
http://dx.doi.org/10.1155/2013/215391
Research Article

Influence of the Toothpaste with Brazilian Ethanol Extract Propolis on the Oral Cavity Health

1Department of Conservative Dentistry with Endodontics, Faculty of Medicine and Dentistry, Medical University of Silesia in Katowice, Plac Akademicki 17, 41902 Bytom, Poland
2Department of Oral Surgery, Faculty of Medicine and Dentistry, Medical University of Silesia in Katowice, Plac Akademicki 17, 41-902 Bytom, Poland
3Department of Microbiology and Immunology, Faculty of Medicine and Dentistry, Medical University of Silesia in Katowice, Ul Jordana 19, 41-808 Zabrze, Poland
4Nihon Natural Therapy Research Laboratory, 6-26-12 Nishishinjuku, Shinjuku-ku, 160-0023 Tokyo, Japan
5Nippon Zettoc Research Laboratory, 3-26 Kudan-Minami 2-Chome, Chiyoda-ku, 102-0074 Tokyo, Japan
6Institute of Technical Biochemistry, Faculty of Biotechnology and Food Sciences, Lodz Technical University Ul Stefanowskiego 4/10, 90-924 Łódź, Poland
7Department of Chemistry, Faculty of Medicine and Dentistry, Medical University of Silesia in Katowice, Ul Jordana 19, 41-808 Zabrze, Poland

Received 29 March 2013; Accepted 20 April 2013

Academic Editor: Andrzej K. Kuropatnicki

Copyright © 2013 Dariusz Skaba et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Propolis-based therapeutic agents represent this potential for the development of new drugs in dental care. The aim of a clinical-cohort study was to determine the influence of application of toothpaste enriched with Brazilian extract of propolis (EEP) on health status of oral cavity. Laboratory analysis was conducted in order to assess the chemical composition of EEP including total phenolic compounds, the DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging activity, ABTS radical cation scavenging activity, and FRAP assay. Clinical research involved two groups of subjects comprising 32 adult patients, with assessment based on the preliminary evaluation of the state of their marginal periodontium. The investigation of oral health indices API, OHI, and SBI and microbiological examination of oral microflora were also carried out. Results obtained indicated time-dependent microbial action of EEP at 50 mg/L concentration, with antimicrobial activity against Gram-positive bacteria. The total decrease of API, OHI, and SBI mean values was observed. Hygienic preparations with 3% content of Brazilian ethanol extract of green propolis (EEP) efficiently support removal of dental plaque and improve the state of marginal periodontium.