Research Article

Oyaksungisan, a Traditional Herbal Formula, Inhibits Cell Proliferation by Induction of Autophagy via JNK Activation in Human Colon Cancer Cells

Figure 1

HPLC fingerprints of OY. (a) HPLC profiling of standard components of constituent herbs contained in OY. (b) Identification of components in OY prescription by HPLC. HPLC chromatograms of components were combined after monitoring at 250, 280, and 320 nm, respectively. Ephedrine, ferulic acid, hesperidin, 6-gingerol, imperatorin, and glycyrrhizin were determined as constituents of Ephedra Herb, Cnidii Rhizoma, Aurantii Fructus, Zingiberis Rhizoma, Angelica Dahurica Root, and glycyrrhizin from Glycyrrhizae Radix, respectively. The retention times of standards for the constituent herbs of OY were detected at 12.86, 22.52, 26.75, 45.02, 47.39, and 57.22 min. Five components in OY were detected at 12.76, 22.43, 26.62, 44.96, and 57.13 min.
231874.fig.001a
(a)
231874.fig.001b
(b)