Oyaksungisan, a Traditional Herbal Formula, Inhibits Cell Proliferation by Induction of Autophagy via JNK Activation in Human Colon Cancer Cells
Figure 4
Identification of the relationship between MAPK activation and autophagy induction by treatment of OY in HCT116 cells. (a) HCT116 cells were treated with 500 μg/mL OY for 0.5, 1, and 6 h. Activation of MAPKs was determined through the detection of phosphorylated protein levels by Western blot analysis. β-Actin was used as a loading control. (b) Cells were pretreated with PD98059 (10 μM), SB203580 (10 μM), or SP60125 (10 μM) for 30 min and added OY at concentration of 500 μg/mL OY, and further incubated for 48 h. Cell viability was determined using MTT assay. Values indicate the mean ± SD. * and ** versus untreated and treated cells with OY only, respectively. (c) After treatment with OY (100, 500, or 1000 μg/mL) for 48 h, cell lysates were prepared and Western blot analysis was performed against Bcl-2, Cyt. c, caspase-3, -9, and PARP. β-Actin was used as a loading control.
