Electroacupuncture-Induced Dynamic Processes of Gene Expression Levels of Endogenous Opioid Peptide Precursors and Opioid Receptors in the CNS of Goats

Table 2

Levels of PENK gene expression in the CNS of goats stimulated by EA (2^−ΔΔCt, mean ± SD, 𝑛=6).

Nuclei and areas	Sham control	−0.5 h	0 h	2 h	4 h	6 h	8 h	12 h	24 h
ACB	1.06 ± 0.05^d	1.00 ± 0.00^d	1.15±0.12^cd	1.29±0.18^cd	1.68±0.21^b	2.01±0.24^a	1.43±0.17^bc	1.17±0.16^cd	1.23±0.14^cd
CAU	1.03 ± 0.02^d	1.00 ± 0.00^d	1.11±0.10^cd	1.26±0.26^bcd	1.75±0.21^ab	2.04±0.19^a	1.26±0.31^bcd	1.49±0.35^bcd	1.54±0.48^abc
AMY	1.17 ± 0.08^e	1.00 ± 0.00^e	1.35±0.19^cd	1.41±0.11^cd	1.57±0.13^bc	1.31±0.12^d	1.74±0.06^b	2.10±0.10^a	1.51±0.13^cd
PVH	1.06 ± 0.04^e	1.00 ± 0.00^e	1.31±0.09^d	1.38±0.11^cd	1.89±0.12^b	2.34±0.10^a	1.57±0.13^c	2.06±0.19^b	1.53±0.15^cd
VMH	1.24 ± 0.16^e	1.00 ± 0.00^e	1.29±0.06^de	1.47±0.10^cd	1.69±0.17^bc	2.42±0.18^a	1.44±0.21^cd	1.63±0.15^bc	1.87±0.20^b
PAG	1.22 ± 0.21^f	1.00 ± 0.00^f	1.64±0.15^ef	2.46±0.37^cd	2.86±0.37^ab	3.37±0.41^a	2.68±0.43^bc	2.02±0.36^de	2.22±0.33^cde
DR	1.17 ± 0.08^d	1.00 ± 0.00^d	1.37±0.09^cd	1.55±0.05^bcd	1.98±0.22^ab	2.42±0.42^a	1.93±0.43^ab	1.61±0.37^bc	1.75±0.31^bc
HB	1.05 ± 0.04^f	1.00 ± 0.00^f	1.22±0.07^def	1.29±0.17^cde	1.54±0.21^bc	1.16±0.07^ef	1.48±0.16^bcd	1.72±0.14^ab	1.93±0.18^a
PBN	1.16 ± 0.07^e	1.00 ± 0.00^e	1.37±0.10^de	1.50±0.23^cd	1.77±0.31^bc	2.21±0.25^a	1.44±0.15^cd	1.67±0.21^bcd	1.89±0.19^ab
GI	1.05 ± 0.09^e	1.00 ± 0.00^e	1.27±0.06^d	1.39±0.17^cd	1.61±0.12^c	1.93±0.18^b	2.39±0.20^a	1.59±0.11^c	1.33±0.11^d
SOL	1.08 ± 0.12^e	1.00 ± 0.00^e	1.33±0.11^d	1.49±0.21^bcd	1.71±0.21^b	2.12±0.16^a	1.36±0.13^cd	1.52±0.17^bcd	1.65±0.22^bc
NH	1.16 ± 0.04^e	1.00 ± 0.00^e	1.54±0.13^d	2.17±0.22^c	3.22±0.13^b	4.27±0.43^a	2.19±0.37^c	1.59±0.24^d	2.17±0.23^c

There was difference (𝑃<0.05) between the values with different letters, and no difference (𝑃>0.05) with the same letters in a line. The letters in the following tables have the same meanings.