Tetrandrine Suppresses Cancer Angiogenesis and Metastasis in 4T1 Tumor Bearing Mice
Figure 2
TET inhibits cell adhesion (a), migration (b), and invasion (c) in HUVECs. (a) Cell adhesion assay. HUVECs and 4T1 cells were used to study the cell adhesive ability. ** versus vehicle control. (b) Cell migration assay. Wound-healing model was used for evaluation of cell migration ability. Three random views were photographed along the scraped line in each well at 100x magnification. (c) Cell invasion assay. Cell invasive ability was measured with a transwell system with a polycarbonate membrane (8 μm pores). Images were captured at 200x magnification. (d) Effects of TET on secretion of matrix metalloproteinase-2 (MMP-2). Gelatin zymography was carried out in an SDS-PAGE gel that contained 0.1% gelatin. (e) Effect of TET on ESM-1, ICAM-1, and integrin β5 expressions in tumor tissues. 4T1 tumor bearing mice (top panel) and TET treated tumor bearing mice (bottom panel) (magnification ×400).
