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Evidence-Based Complementary and Alternative Medicine
Volume 2013, Article ID 269874, 7 pages
http://dx.doi.org/10.1155/2013/269874
Research Article

Ethanol Extract of Ulmus pumila Root Bark Inhibits Clinically Isolated Antibiotic-Resistant Bacteria

1Department of Oral Biochemistry, School of Dentistry, Wonkwang University, Iksan 570-749, Republic of Korea
2Wonkwang Research Institute for Food Industry, Iksan 570-749, Republic of Korea
3College of Education, Wonkwang University, Iksan 570-749, Republic of Korea
4Department of Oral Microbiology, School of Dentistry, Wonkwang University, Iksan 570-749, Republic of Korea

Received 1 August 2013; Accepted 3 September 2013

Academic Editor: Tong Ho Kang

Copyright © 2013 Yong-Ouk You et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

In this study, root bark of Ulmus pumila (U. pumila) was extracted with ethanol, and then the antimicrobial effects were tested on clinically isolated 12 MRSA strains and 1 standard MRSA strain. U. pumila showed antibacterial activities against all MRSA strains. Minimum inhibitory concentration (MIC) of U. pumila root bark against all MRSA strains revealed a range from 125 to 250 μg/mL. These results may provide the scientific basis on which U. pumila root bark has traditionally been used against infectious diseases in Korea. In real-time PCR analysis, the sub-MIC (64–125 μg/mL) concentrations of U. pumila root bark extract showed the inhibition of the genetic expressions of virulence factors such as mecA, sea, agrA, and sarA in standard MRSA. Phytochemical analyses of U. pumila root bark showed relatively strong presence of phenolics, steroids, and terpenoids. These results suggest that the ethanol extract of U. pumila root bark may have antibacterial activity against MRSA, which may be related to the phytochemicals such as phenolics, steroids, and terpenoids. Further studies are needed to determine the active constituents of U. pumila root bark responsible for such biomolecular activities.