Bioactive Lipidic Extracts from Octopus (Paraoctopus limaculatus): Antimutagenicity and Antiproliferative Studies

Table 4

Anti-mutagenic potential of AFB₁^a-spiked fractions obtained from thin layer chromatography fractionation of fraction RB21 (𝑅_𝑓 = 0.78–1.00) from TLC 2 (average^b TA100 and TA98 revertants/plate, with S9).

Extract fraction^c	Fraction dilution^c
	1×10^0	1×10^−1	1×10^−2	1×10^−3
TA98
RB211 (𝑅𝑓 = 0.00–0.22)	628±49	576±43	499±55	549±34
RB212 (𝑅𝑓 = 0.22–0.68)	371±13	345±25	489±29	650±68
RB213 (𝑅𝑓= 0.68–1.00)	199±15	335±23	401±28	576±21
TA100
RB211 (𝑅𝑓 = 0.00–0.22)	1549±89	1466±123	1657±220	1425±124
RB212 (𝑅𝑓 = 0.22–0.68)	1006±93	941±45	1178±129	918±28
RB213 (𝑅𝑓 = 0.68–1.00)	499±35	829±63	1237±128	1465±121

^aAFB₁ (used as positive control) tested at 500 ng/plate induced 605 ± 33 and 1689 ± 78 revertants/plate for TA98 and TA100, respectively. Spontaneous revertants were 55±3 and 188±14 for TA98 and TA100, respectively.
^bValues are means of three replicates ± SEM.
^cExtracts were diluted and spiked with enough toxin to yield 500 ng of pure AFB₁/plate.