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Evidence-Based Complementary and Alternative Medicine
Volume 2013, Article ID 273682, 10 pages
http://dx.doi.org/10.1155/2013/273682
Research Article

Osthole Improves Spatial Memory Deficits in Rats via Hippocampal -Adrenergic and D1/D2 Receptors

1The School of Chinese Medicines for Post-Baccalaureate, I-Shou University, No.8, Yida Road, Yanchao Township, Kaohsiung County 82445, Taiwan
2The Department of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, College of Pharmacy, China Medical University, No.91, Hsueh Shih Road, Taichung 40402, Taiwan
3Department of Cosmeceutics, College of Pharmacy, China Medical University, No.91, Hsueh Shih Road, Taichung 40402, Taiwan
4Department of Nutrition, China Medical University, No.91, Hsueh Shih Road, Taichung 40402, Taiwan
5Department of Health, Taichung Hospital, The Executive Yuan, No.199, San Min Road, Taichung 40403, Taiwan

Received 25 October 2012; Accepted 29 January 2013

Academic Editor: Jae Youl Cho

Copyright © 2013 Li-Wei Lin et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The present study evaluated the effect of osthole, an active ingredient isolated from Cnidium monnieri L. Cusson, on spatial memory deficits caused by central neurotoxins using the Morris water maze in rats. The involvement of catecholaminergic receptors on the memory-enhancing effect of osthole in rat hippocampus was further investigated by intrahippocampal injection of catecholaminergic receptor antagonists. Intracisternal injection of osthole (10 μg/brain) improved the spatial performance and working memory impairments caused by the catecholaminergic neurotoxin 6-hydroxydopamine. No significant differences in swimming speeds were observed among sham, neurotoxin-induced, and osthole-treated groups. Intracisternal osthole injection also attenuated the spatial performance and working memory impairments caused by the receptor antagonist phenoxybenzamine, the D1 receptor antagonist SCH 23390, and the D2 receptor antagonist sulpiride. Therefore, we demonstrated that the effect of osthole on improving spatial memory deficits may be related to the activation of hippocampal and D1/D2 receptors.