A Small Molecule Swertisin from Enicostemma littorale Differentiates NIH3T3 Cells into Islet-Like Clusters and Restores Normoglycemia upon Transplantation in Diabetic Balb/c Mice

Table 1

List of antibodies used for flow cytometry, immunohistochemistry, and western blotting.

Name of antibody	Company	Target against species	Mono/polyclonal	Molecular weight (kDa)	Source	Dilution for WB	Dilution for ICC	Dilution for flow cytometry
Beta actin	Thermo scientific	Hu, Rt, Mu	Mono	42	Mouse	1 : 5000	NA	NA
p-Smad-2	Cell signalling	Rt, Mu	Poly	60	Rabbit	1 : 1000	NA	NA
Smad-7	R & D systems	Rt, Mu	Mono	50	Mouse	1 : 1000	1 : 100	NA
Nestin	Sigma-Aldrich	Hu, Rt, Mu	Mono	177	Rabbit	1 : 1000	1 : 250	NA
Pdx1	Cell signaling	Hu, Rt, Mu	Mono	42	Rabbit	1 : 1000	1 : 250	NA
Vimentin	Sigma	Hu, Rt, Mu	Mono	53	Mouse	1 : 1000	1/250	NA
CD90-FITC	BD	Hu, Mu	Mono	—	Mouse	—	1 : 100	1 : 10
CD34-FITC	BD	Hu, Mu	Mono	—	Mouse	—	1 : 100	1 : 10
CD45-APC	BD	Hu, Mu	Mono	—	Mouse	—	1 : 100	1 : 10
CD49b-FITC	BD	Hu, Rt, Mu	Mono	—	Mouse	—	1 : 100	1 : 10
CD117-PE	BD	Hu, Rt, Mu	Mono	—	Mouse	—	1 : 100	1 : 10
CD44-PE	BD	Hu, Rt, Mu	Mono	—	Mouse	—	1 : 100	1 : 10
C-peptide	Cell signalling	Hu, Rt, Mu	Poly	4	Rabbit	—	1 : 100	NA
Insulin-Alexa 548	Santa Cruz	Hu, Rt, Mu	Mono	6	Guinea Pig	—	1 : 200	NA
Glucagon	Sigma	Hu, Rt, Mu	Mono	3.5	Mouse	—	1 : 200	NA
Somatostatin	Sigma	Hu, Mu	Poly	12	Rabbit	—	1 : 200	NA