A Small Molecule Swertisin from Enicostemma littorale Differentiates NIH3T3 Cells into Islet-Like Clusters and Restores Normoglycemia upon Transplantation in Diabetic Balb/c Mice

Table 2

List of primer sequences of RT-PCR with annealing temperature and amplicon size.

Name of gene	Gene accession number	Primer sequence forward	Primer sequence reverse	PCR conditions (Tm)	Amplicon size (BP)
α-SMA	NT_039687.7	AGTCGCCATCAGGAACCTCGAG	ATCTTTTCCATGTCGTCCCAGTTG	60	296
Vimentin	NT_039202.7	AGCGGGACAACCTGGCCG	GGGAAGAAAAGGTTGGCAGAGGC	58	744
Nestin	NT_039240.7	GCGGGGCGGTGCGTGACTAC	AGGCAAGGGGGAAGAGAAGGATGT	58	326
PDX-1	NT_039324.7	CTC GCT GGG AAC GCT GGA ACA	GCT TTG GTG GAT TTC ATC CAC GG	55	229
Ngn-3	NT_039500.7	ACTAGGATGGCGCCTCATCCCTTG	GGTCTCTTCACAAGAAGTCTGAGA	57	658
Pax-4	NM_011038.2	TGGCTTCCTGTCCTTCTGTGAGG	TCCAAGACTCCTGTGCGGTAGTAG	62	214
Nkx-6.1	NM_144955.2	ATGGGAAGAGAAAACACACCAGAC	TAATCGTCGTCGTCCTCCTCGTTC	60	280
Insulin	NM_008386.3	GCCCAGGCTTTTGTCAAACA	CTCCCCACACACCAGGTAGAG	55	90
Glucagon	NM_008100.3	ATGAAGACCATTTACTTTGTGGCT	GGTGTTCATCAACCACTGCAC	58	243
Reg-1	NM_009042.1	AAGCTGAAGAAGACCTGCCA	TGTTAGGAGACCCAGTTGCC	60	123
GAPDH	NT_166349.1	CAAGGTCATCCATGACAACTTTG	GTCCACCACCCTGTTGCTGTAG	58	496
List of primer sequences for Q-PCR with annealing temperature and amplicon size
PDX-1	NT_039324.7	ACTTGAGCGTTCCAATACGG	GCTTTGGTGGATTTCATCCACGG	55	813
INS-2	NM008387	TGTCTCTGGGGAAATGGGATTC	TGCTGCTTGACAAAAGCCTG	59	243