Primo Vascular System in the Subarachnoid Space of a Mouse Brain

Figure 4

(a) Another specimen of the PN located in the circle shown in the stereomicroscopic image of the dura mater and the pia mater of a mouse brain (sample no. 11 in Table 1). It was located 4,800 μm from the λ point. (b) Phase-contrast microscope images of the PN. It had a fusiform shape, and its size was 200 μm × 400 μm. (c) and (d) Fluorescence images of the PN with DAPI and phalloidin staining, respectively. (e) and (f) Fluorescence images of the PV attached to the PN with DAPI and phalloidin staining, respectively. The pia mater that covered the PV was removed to reveal the PV. Rod-shaped nuclei and f-actin distributions were noticed.