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Evidence-Based Complementary and Alternative Medicine
Volume 2013, Article ID 287803, 11 pages
Research Article

Immunomodulatory Activity of Lactococcus lactis A17 from Taiwan Fermented Cabbage in OVA-Sensitized BALB/c Mice

1Department of Science Education, National Taipei University of Education, 134, Section 2, Heping E. Road, Da-an District, Taipei 10671, Taiwan
2Institute of Biochemistry and Molecular Biology, National Yang-Ming University, 155, Section 2, Linong Street, Taipei 11221, Taiwan
3Asian Probiotics and Prebiotics Corporation, Room 9B, No. 6-8 Lane 511, Wuzhong Road, Minhang District, Shanghai, China
4GINCARE Int'l Enterprises Co., Ltd., No. 24, Section 3, Chungyang Road, Tu-Cheng District, New Taipei City 23673, Taiwan

Received 24 October 2012; Accepted 17 December 2012

Academic Editor: Jae Youl Cho

Copyright © 2013 Hui-Ching Mei et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


From fermented Taiwan foods, we have isolated numerous lactic acid bacteria (LAB) of plant origin and investigated their biological activities. This study aimed to investigate the immunomodulatory effect and mechanism of Lactococcus lactis A17 (A17), isolated from Taiwan fermented cabbage, on ovalbumin (OVA)-sensitized mice. Human peripheral blood mononuclear cells were used to verify immune responses of A17 by IFN-γ production. Live (A17-A) and heat-killed A17 (A17-H) were orally administered to OVA-sensitized BALB/c mice to investigate their effects on immunoglobulin (Ig) and cytokine production. The mRNA expression of Toll-like receptors (TLR) and nucleotide binding oligomerization domain (NOD)-like protein receptors in spleen cells was analyzed by real-time RT-PCR. Both live and heat-killed A17 modulate OVA-induced allergic effects. B-cell response was modulated by diminishing IgE production and raising OVA-specific IgG2a production, while T-cell response was modulated by increasing IFN-γ production and decreasing IL-4 production. The mRNA expression of NOD-1, NOD-2, and TLR-4 was down-regulated by A17 as well. This is the first report to describe a naïve Lactococcus lactis A17 strain as a promising candidate for prophylactic and therapeutic treatments of allergic diseases via oral administration. Our results suggest the ameliorative effects of A17 may be caused by modulating NOD-1 NOD-2, and TLR-4 expression.