Spinal Serotonergic and Opioid Receptors Are Involved in Electroacupuncture-Induced Antinociception at Different Frequencies on ZuSanLi (ST 36) Acupoint
Diagram of intrathecal cannulation. A middle lumbar skin incision was performed, following the paravertebral muscle detached from the spinal process and retracted laterally. L5 spinal process was removed then opened the dura for exposing the spinal cord. The dura was perforated with a short bevel of no. 30 gauze needle following inserted 2 cm PE-10 into the subarachnoid space (SAS). A drop of cyanoacrylic glue was added for anchoring the PE-10 besides the L6 spinal process. The operation wound was irrigated with normal saline and closed in layers with silk streaks, no. 4.