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Evidence-Based Complementary and Alternative Medicine
Volume 2013, Article ID 293080, 7 pages
http://dx.doi.org/10.1155/2013/293080
Research Article

GC/MS Evaluation and In Vitro Antioxidant Activity of Essential Oil and Solvent Extracts of an Endemic Plant Used as Folk Remedy in Turkey: Phlomis bourgaei Boiss.

1Department of Gastronomy and Culinary Arts, Faculty of Tourism, University of Necmettin Erbakan, Meram, Konya 42100, Turkey
2Department of Chemistry, Faculty of Science and Literature, Celal Bayar University, Muradiye, Manisa 45030, Turkey
3Department of Chemistry, Faculty of Arts and Sciences, Kilis 7 Aralık University, Kilis 79000, Turkey
4Department of Chemistry, Faculty of Science, Atatürk University, Erzurum 25240, Turkey

Received 10 February 2013; Accepted 12 April 2013

Academic Editor: F. R. F. Nascimento

Copyright © 2013 Cengiz Sarikurkcu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

This study was outlined to examine the chemical composition of hydrodistilled essential oil and in vitro antioxidant potentials of the essential oil and different solvent extracts of endemic Phlomis bourgaei Boiss. used as folk remedy in Turkey. The chemical composition of the oil was analyzed by GC and GC-MS, and the predominant components in the oil were found to be β-caryophyllene (37.37%), (Z)-β-farnesene (15.88%), and germacrene D (10.97%). Antioxidant potentials of the solvent extracts and the oil were determined by four testing systems including β-carotene/linoleic acid, DPPH, reducing power, and chelating effect. In β-carotene/linoleic acid assay, all extracts showed the inhibition of more than 50% at all concentrations. In DPPH, chelating effect, and reducing power test systems, the water extract with 88.68%, 77.45%, and 1.857 (absorbance at 700 nm), respectively, exhibited more excellent activity potential than other extracts (hexane, ethyl acetate and methanol) and the essential oil at 1.0 mg/mL concentration. The amount of the total phenolics and flavonoids was the highest in this extract (139.50 ± 3.98 μg gallic acid equivalents (GAEs)/mg extract and 22.71 ± 0.05 μg quercetin equivalents (QEs)/mg extract).