Evidence-Based Complementary and Alternative Medicine / 2013 / Article / Fig 1

Research Article

Antcin C from Antrodia cinnamomea Protects Liver Cells Against Free Radical-Induced Oxidative Stress and Apoptosis In Vitro and In Vivo through Nrf2-Dependent Mechanism

Figure 1

Protective effects of antcin C on AAPH-induced cell death and ROS generation in HepG2 cells. (a) Chemical structure of antcin C. (b) and (d) Cells were pretreated with increasing concentrations of antcin C (5–20 μM), silymarin (100 μM), or NAC (1 mM) for 2 h; then oxidative stress was induced by AAPH (10 mM) for 24 h. Cell viability was measured by MTT assay. Percentage of viable cells was calculated against that of control cells. (c) Cells were pretreated with antcin C (5–20 μM) or silymarin (100 μM) for 2 h, and then ROS generation was induced by AAPH (10 mM) for 30 min. The percentage of fluorescence intensity of the DCF stained cells was quantified using a fluorescence spectrophotometer as described in Materials and Methods. Values represent the mean ± SD of three independent experiments. , , and were considered significant for sample versus AAPH. was considered significant for control versus AAPH.