Antcin C from Antrodia cinnamomea Protects Liver Cells Against Free Radical-Induced Oxidative Stress and Apoptosis In Vitro and In Vivo through Nrf2-Dependent Mechanism

Figure 6

Antcin C upregulates antioxidant genes in AAPH-challenged liver tissues. Mice were pretreated with antcin C (25–200 mg/kg) or silymarin (100 mg/kg) for 5 days, and then challenged with a single dose of AAPH (80 mg/kg). Mice were sacrificed, and the liver sections were subjected to immunohistochemistry to analyse the protein expression levels of HO-1, NQO-1, and Nrf2 using specific antibodies. The relative intensity of antibody positive staining cells was quantified by Image-Pro Plus software. The results are presented as the mean ± SD of three independent experiments. ^∗𝑃<0.05, ^∗∗𝑃<0.01, and ^∗∗∗𝑃<0.001 were considered significant for sample versus control.

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