Antrodia camphorata Grown on Germinated Brown Rice Suppresses Melanoma Cell Proliferation by Inducing Apoptosis and Cell Differentiation and Tumor Growth
Figure 3
(a) Melanin production in B16F10 melanoma cells upon CBR EtOAc treatment (0, 1, 5, and 10 μg/mL) for 48 hours. The melanin content was measured with a microplate reader at 475 nm. All experiments are performed in triplicate (). (b) Representative western blot images of MITF, TRP-1, and β-actin under EtOAc fraction treatment (0, 1, 5, and 10 μg/mL). (c) Phase contrast microscopy: representative images of B16F10 melanoma cells treated with 0, 1, 5, and 10 μg/mL CBR EtOAc and controls for 48 hours (scale bar = 200 μm).
