Gross Morphological Features of the Organ Surface Primo-Vascular System Revealed by Hemacolor Staining
Figure 2
Images of the whole tissue and cells of the PVS stained by Hemacolor. (a) The unstained whole PVS sample in Krebs solution. (b) Typical unsectioned longitudinal image of a whole PVS tissue composed of PNs and a PV. Note the continuous inner space structures (dotted circles) along the longitudinal axis of the PVS. There are two spaces (dotted circles, PN1, 30–50 μm; PN2, 10–50 μm) in the PNs and one space (dotted circle in bottom inset, 20–30 μm) in the PV. PVS cells at the edges were more abundant than in the middle of the PV. (b1) Distribution of the cells in the inner region (marked as “b1” in (b)) of the PNs. Note that most PN cells (arrow, large granular cells; arrowhead, small round cells) are round and are placed evenly and randomly. (b2) Distribution of the cells in the inner region (marked as “b2” in (b)) of the PV. Note that most PV cells (arrow, large granular cells; arrowhead, small round cells) are elliptical and horizontally arranged along the long axis of the PV. (b3 and b4) Distribution of the cells in the inner spaces (marked as “b3” and “b4” in (b)) of the PV. Note that the inner space (arrows) also contains numerous PVS cells (arrowhead, small round cells; open arrowhead, small yellowish cells; dotted circle, granules).
