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Evidence-Based Complementary and Alternative Medicine
Volume 2013, Article ID 350815, 12 pages
http://dx.doi.org/10.1155/2013/350815
Research Article

Gross Morphological Features of the Organ Surface Primo-Vascular System Revealed by Hemacolor Staining

1Laboratory of Veterinary Pharmacology, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 151-742, Republic of Korea
2Laboratory Animal Research Center, College of Pharmacy, Hanyang University, Ansan 426-791, Republic of Korea
3Laboratory of Clinical Pathology, College of Veterinary Medicine, Seoul National University, Seoul 151-742, Republic of Korea

Received 11 February 2013; Revised 30 June 2013; Accepted 2 July 2013

Academic Editor: Walter J. Akers

Copyright © 2013 Chae Jeong Lim et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Movie S1: Whole primo-vessel tissue was stained with acridine orange and the 3D microscopy was performed by a confocal laser scanning microscope (LSM710, Carl Zeiss, Germany) to show an inner space part within the primo-vessel. Most cells of the primo-vessel were stained green color (denoting DNA), and the inner space with low cellularity appeared dark along the longitudinal axis of the primo-vessel.

Movie S2: Spontaneous movement of granules within a putative mast cell in the primo-node slice was recorded under a light microscope with differential interference contrast (BX50WI, Olympus, Tokyo, Japan) using a USB digital CCD camera series 150PIII.

Movie S3: Spontaneous movement of a granule in isolation was recorded under a light microscope with differential interference contrast (BX50WI, Olympus, Tokyo, Japan) using a USB digital CCD camera series 150PIII.

  1. Supplementary Movie S1
  2. Supplementary Movie S2
  3. Supplementary Movie S3