Figure 3: (a) Normalized ATP content in supernatant from untreated cells (Control) and of cells after having been treated for 5 min with red laser light (Irradiation). Data were normalized to the controls of the respective batch of cells and represent averages of 8 determinations (± SEM). One corresponds to 6.0 nM/106 cells. (b) Relative increase in intracellular Ca2+ in response to 1 and 5 min of red laser light compared to control cells; measurements were performed with cells untreated (Irradiation) and cell treated for one min with EGTA (+EGTA). The data represent averages ±SEM ( 15–17). *Significant difference compared to control.