Evidence-Based Complementary and Alternative Medicine / 2013 / Article / Fig 7

Research Article

Evaluation of In Vitro Anti-Inflammatory Activities and Protective Effect of Fermented Preparations of Rhizoma Atractylodis Macrocephalae on Intestinal Barrier Function against Lipopolysaccharide Insult

Figure 7

The effect of treatment with LPS in combination with saline (LC) or with two different concentrations of fermented Rhizoma Atractylodis Macrocephalae-1 (FRAM-1) and fermented Rhizoma Atractylodis Macrocephalae-2 (FRAM-2) formulations on expression of TNF-α (a), IL-1β (b), and IL-6 (c) genes in RAW264.7 cells. The cells were treated with the indicated concentrations of FRAMs for 24 h. The control cells (N) and the noncontrol cells that were assigned to treatment with LPS alone (LC) were treated with sterile saline instead of herbal extracts. After this treatment, the noncontrol and control cells were treated with LPS (10 μg/mL) and PBS, respectively, for 24 h, followed by determination of gene expression of the above-mentioned cytokines. The detailed treatment regimen and experimental conditions are described in Section 2. The values are expressed as the mean ± SD, . The data for expression of all genes were log-transformed prior to analysis by ANOVA. significant difference compared to control cells ( ); significant difference compared to cells treated with LPS plus saline ( ); lower compared to all other treatment groups ( ). FHE: fermented herbal extract.

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