Reconstitution of Kidney Side Population Cells after Ischemia-Reperfusion Injury by Self-Proliferation and Bone Marrow-Derived Cell Homing

Figure 1

Depletion of SP cells in the ischemic kidney after IRI. (a) Fluorescence-activated cell sorting isolation of SP and non-SP cells in the left kidney from normal animals and IRI animals. Hoechst low SP cells are identified by the R3 area and demonstrate an a decrease in kidney SP cells within 1 day after IRI. (b) The histological score of kidney (HSK) in kidneys from sham-operated and IRI-mice was calculated. ^∗𝑃<0.05 versus sham; ^†𝑃<0.05 versus IRI 1 d. ((c) and (d)) The SP cells in left (c) and right (d) kidneys from sham-operated (white bars) and IRI (black bars) animals. ^∗𝑃<0.05 versus sham; ^†𝑃<0.05 versus IRI 1 d. (e) In vitro apoptosis analysis of kidney SP and non-SP cells in basal conditions and after simulated ischemia. Cultured kidney SP and non-SP cells were subjected to hypoxia and SD alone or in combination for 6 h. Bar graph described from the FACS-based Annexin V/propidium iodide apoptosis assay. The cells without both hypoxia and SD stimulation were used as controls. ^∗𝑃<0.05 versus control; ^†𝑃<0.05 versus hypoxia; ^‡𝑃<0.05 versus SD; ^#𝑃<0.05 versus non-SP.

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