Shikonin Suppresses Human T Lymphocyte Activation through Inhibition of IKKβ Activity and JNK Phosphorylation
Figure 4
Effect of shikonin on CD25, CD69, and CD71 expression on human T lymphocytes. Human T lymphocytes (106) were pretreated with shikonin for 2 h and then stimulated by PMA (20 ng/mL)/ionomycin (1 μM) (P/I) for 48 h or 72 h, respectively. The cells were double stained with PE-CD3 and FITC-CD69 (a), PE-CD3 and FITC-CD25 (b), PE-CD3 or FITC-CD71 (c) antibodies and then analyzed by flow cytometry. The unstimulated cells were served as negative control. Values represent percentages of the double stained cells.
