The Effect of Electroacupuncture on Osteosarcoma Tumor Growth and Metastasis: Analysis of Different Treatment Regimens
Representative photomicrographs of fluorescent immunohistochemical staining of lymphatics (LYVE1) in the hind paw tumor taken at 200x (a) and 600x (b), respectively. The images were captured with an MRC 1024 Confocal/Multiphoton Imaging System (Bio-Rad, Hercules, CA). Scale bars represent 50 μm. (c) A graph showing the effect of EA-2X/3 and EA-2X/7 treatment on lymphatic immunostaining density at the tumor site. EA-2X/3, but not EA-2X/7, displayed a robust decrease in peripheral lymphatic density when compared to both its Tumor-No EA (TUM) and EA-2X/7 counterparts (/group). Positive LYVE1 (lymphatic) immunostaining is indicated by the single arrows.
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