The Effect of Electroacupuncture on Osteosarcoma Tumor Growth and Metastasis: Analysis of Different Treatment Regimens
Figure 5
Representative photomicrographs of H&E stained lung sections showing the presence of visible pulmonary tumors (arrows) at ((a)-(b)) 4x, ((c)-(d)) 10x, and ((e)-(f)) 40x magnifications in animals receiving twice-weekly EA ((a), (c), and (e)) or Tumor-No EA ((b), (d), (f)). Nikon ACT-1 (Nikon, Melville, NY) software was used to acquire images for processing, and tumor quantity and area were measured per section and totaled per animal using MetaMorph software (Molecular Devices, Sunnyvale, CA). EA-2X/3 significantly reduced the average tumor area present in the transverse lung sections ((a)/(c)/(e) compared to (b)/(d)/(f)). Scale bars represent ((a)-(b)) 500 μm, ((c)-(d)) 200 μm, and ((e)-(f)) 100 μm.
