Evidence-Based Complementary and Alternative Medicine / 2013 / Article / Fig 1

Research Article

The Methanol Extract of Angelica sinensis Induces Cell Apoptosis and Suppresses Tumor Growth in Human Malignant Brain Tumors

Figure 1

The cytotoxic effects of AS-M on GBM cells. (a) The methanol extraction procedure for the roots of Angelica sinensis. (b) AS-M induced cell apoptosis. After 72 h of treatment, apoptotic cells were detected by fluorescence microscopy (×400) using in situ TUNEL staining and propidium iodide counterstaining. (c) AS-M enhanced cell cycle arrest at the G0-G1 phase. (d) AS-M increased the sub-G1 cells. DBTRG-05MG cells were treated with AS-M or control medium for 0, 24, 48, and 72 hours, washed, and stained with propidium iodide. Each column represents the mean ± SD; .

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