Evidence-Based Complementary and Alternative Medicine / 2013 / Article / Fig 4

Research Article

The Methanol Extract of Angelica sinensis Induces Cell Apoptosis and Suppresses Tumor Growth in Human Malignant Brain Tumors

Figure 4

AS-M suppressed the growth of the human GBM tumors by inducing cell apoptosis, cell cycle arrest, and antiangiogenesis in vivo. (a) Immunohistochemical staining and the TUNEL assay were performed on the GBM tumor tissues. Representative photographs of sections from the control group and AS-M-treated group are shown. In the immunohistochemistry experiment, GBM tumors were stained for the cell proliferation marker Ki-67 and the cell apoptosis marker cleaved caspase-3. In the TUNEL assay, the tumors were stained to show the DNA fragmentation of apoptotic cells. The Ki-67- and caspase-3-positive cells are shown in brown, and the TUNEL-positive cells are shown in green (×400). Quantification of protein expression was performed using Quick score, which multiplies the staining intensity by the percentage of positive cells. (b) The mean values for the Ki-67, caspase-3, VEGF, VEGFR1, and VEGFR2 levels and the vessel number were calculated for the tumors treated with AS-M. All the data in AS-M group is significant difference from the control group ( ).

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