The Herbal Drug Melampyrum pratense L. (Koch): Isolation and Identification of Its Bioactive Compounds Targeting Mediators of Inflammation

Figure 1

HPLC-ELDS-LT chromatogram of the methanolic extract of Melampyrum pratense. Analyses were performed on an Acclaim 120 C₁₈ reversed-phase column (150 mm × 2.1 mm i.d., 3 μm) equipped with an Acclaim 120 C₁₈ guard column (10 mm × 2.1 mm i.d., 5 μm) from Dionex (Germering, Germany) with the following mobile phase gradient of acetonitrile-water (A: H₂O + formic acid (pH = 2.5), B: MeCN): 2% B (5 min), 2%–10% of B in 11 min, 10%–20% of B in 24 min, 20%–50% of B in 20 min, and 50%–95% of B in 5 min at a flow rate of 0.4 mL/min and a temperature set to 35°C. The following compounds were identified: (1) catalpol, (2) aucubin, (3) mussaenosidic/loganic acid, (4) mussaenoside, and (5) melampyroside.