The Herbal Drug Melampyrum pratense L. (Koch): Isolation and Identification of Its Bioactive Compounds Targeting Mediators
of Inflammation
Figure 5
Effect of Melampyrum pratense extracts and their individual components on the induction of IL-8 protein after stimulation with LPS (a) or TNF-α (b). Mela MeOH: methanolic extract, Mela det: detannified methanol extract, and Mela OC 30/OC 70: 30%/70% methanolic SPE subfraction of the chlorophyll-depleted dichloromethane extract. Extracts and fractions were tested at 30 μg/mL, compounds at 30 μM. BAY (5 μM) was used as a positive control. The data are presented as mean values ± standard errors of sixtuplicate measurements. Very similar results were obtained in an independent experiment. Stars indicate statistical significance compared to LPS or TNF-α activation (ANOVA): *, **, and ***.
